NSC-derived extracellular matrix-modified GelMA hydrogel fibrous scaffolds for spinal cord injury repair

Abstract Cell-derived extracellular matrix (ECM) has been applied in spinal cord injury (SCI) regeneration because of its various biological functions. However, insufficient mechanical properties limit wide application. Herein, we developed GelMA/ECM hydrogel fibrous scaffolds (GelMA/ECM scaffolds) that can recruit and enhance the differentiation neural stem cells (NSCs) by electrospinning decellularization techniques. Moreover, had good reinforced cell adhesion proliferation. Compared to GelMA (GelMA scaffolds), promoted more NSCs toward neurons markedly enhancing expression MAP-2 Tuj-1 decreasing GFAP expression. In addition, significantly reduced proportion M1-phenotype macrophages, which is favorable for SCI repair. vivo, recruited at injured site, neuron regeneration, formation glial scars inflammatory response, further led a significant improvement functional recovery SCI. Therefore, this scaffold shows potential regenerative medicine, mainly